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Lutein downregulates retinal vascular endothelial growth factor possibly via hypoxia inducible factor 1 alpha and X-box binding protein 1 expression in streptozotocin induced diabetic rats.

Sharavana, Gurunathan and Baskaran, V. (2017) Lutein downregulates retinal vascular endothelial growth factor possibly via hypoxia inducible factor 1 alpha and X-box binding protein 1 expression in streptozotocin induced diabetic rats. Journal of Functional Foods, 31. pp. 97-103. ISSN 1756-4646

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Abstract

Lutein, a retinal-specific carotenoid, was assessed for angioprotective efficacy via hypoxia inducible factor (HIF1a) and X-box binding protein 1 (XBP1) pathway in diabetic retina. Streptozotocin-induced diabetic rats were orally gavaged with or without lutein (39 nmol/day) for 8 weeks. Lutein mediated regulation of vascular endothelial growth factor (VEGF) and superoxide dismutase 2 (SOD2) was analyzed using qPCR and immunoblotting. VEGF transcription factors, Hif1a and XBP1, in retina of streptozotocin induced diabetic rats were assessed using qPCR. Retinal protein carbonyl and lipid peroxide levels were quantified and transverse section of retina was stained for histopathology. Results indicate that lutein prevented the upregulation of VEGF and downregulation of SOD2 in retina of diabetic rats and also prevented abnormalities in ganglion cell, inner and outer nuclear layer in diabetic retina. This study suggests the anti VEGF property of lutein is perhaps via decrease in the mRNA expression of Hif1a and Xbp1.

Item Type: Article
Uncontrolled Keywords: Diabetes HIF1a Lutein Retinal angiogenesis VEGF XBP1
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Jul 2018 10:39
Last Modified: 04 Jul 2018 10:39
URI: http://ir.cftri.com/id/eprint/13580

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