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Thermal assisted alkaline pretreatment of rice husk for enhanced biomass deconstruction and enzymatic saccharification: Physico-chemical and structural characterization.

Md., Shahabazuddin and Sarat Chandra, T. and Meena, S. and Sukumaran, R. K. and Nandini, P. Shetty and Mudliar, S. N. (2018) Thermal assisted alkaline pretreatment of rice husk for enhanced biomass deconstruction and enzymatic saccharification: Physico-chemical and structural characterization. Bioresource Technology, 263. pp. 199-206. ISSN 0960-8524

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Abstract

Thermal assisted alkaline pretreatment (TAAP) of rice husk (RH) was investigated to facilitate enzymatic saccharification by enhancing the enzyme accessibility to cellulosic components. Statistically guided experiments based on the Box-Behnken design involving four factors viz. biomass loading, particle size, NaOH loading and reaction time was considered for optimization. The maximum sugar yield of 371 mg g−1 biomass was obtained at optimized pretreatment condition [biomass loading (10% w/w), particle size (0.25–0.625 mm), NaOH loading (2% w/w), and reaction time (40 min)]. The TAAP of RH resulted in the efficient removal of lignin (14.9–54% (w/w)) with low hemicellulose solubilization [10.7–33.1% (w/w)] and with a simultaneous increase in cellulose concentration [32.65–51.65% (w/w)]. The SEM analysis indicated increased porosity and biomass disruption during TAAP. The FTIR analysis showed progressive removal of noncellulosic constituents, and XRD analysis revealed an increase in cellulose crystallinity post-TAAP indicating the effectiveness of pretreatment.

Item Type: Article
Uncontrolled Keywords: Rice husk Thermal assisted alkaline pretreatment Lignocellulosic biomass Enzymatic saccharification Box-Behnken design
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization
600 Technology > 08 Food technology > 21 Cereals > 01 Rice
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 02 Jul 2018 06:27
Last Modified: 02 Jul 2018 06:27
URI: http://ir.cftri.com/id/eprint/13550

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