[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Whey protein isolate enrichment attenuates manganese-induced oxidative stress and neurotoxicity in Drosophila melanogaster: Relevance to Parkinson’s disease.

Greeshma, M. and Sriranjini, Venkata Rao and Dr., Muralidhara and Rajini, P. S. (2017) Whey protein isolate enrichment attenuates manganese-induced oxidative stress and neurotoxicity in Drosophila melanogaster: Relevance to Parkinson’s disease. Biomedicine & Pharmacotherapy, 95. pp. 1596-1606.

[img] PDF
Biomedicine & Pharmacotherapy 95 (2017) 1596–1606.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Currently, much emphasis is being laid on understanding the role of nutrition to alleviate neurodegenerative conditions. Here we employed a Drosophila model to test the hypothesis that Whey protein isolate (WPI) enrichment can attenuate Manganese (Mn) induced locomotor phenotype, oxidative stress and neurotoxicity. Mn exposure (0–30 mM) to young (8–10 d old) males/females and aged (30–35 d old) males elicited a concentration- dependent temporal mortality and locomotor deficits. In general, females and aged males were more susceptible than young males. Mn exposure (5–15 mM, 5 d) among male flies, besides induction of oxidative stress (elevated levels of MDA and protein carbonyls with a concomitant decrease in glutathione levels), caused significant perturbations in the activities of antioxidant enzymes and mitochondrial complexes. In the co-exposure paradigm, WPI fortification (0.25–0.5%) markedly ameliorated Mn-induced locomotor phenotype, mortality, oxidative stress and restored the activity of the antioxidant enzymes, cholinergic function, and dopamine levels. In a satellite study, flies given prophylactic treatment (10 d) with WPI were more resistant(evidenced by a lower incidence of mortality and locomotor deficits) to an acute MPP+ challenge suggesting its potential to enhance survival pathways. Collectively, these data suggest the potential of WPI enrichment to attenuate Mn-induced toxic manifestations in the Drosophila model. Based on these findings, we propose that fortification of diet with WPI offers a useful approach to alleviate Mn-induced toxic manifestations and neuronal dysfunctions in humans, especially in occupational settings.

Item Type: Article
Uncontrolled Keywords: Manganese Oxidative stress Neurotoxicity Whey protein isolate Drosophila
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization
600 Technology > 08 Food technology > 27 Dairy products
Divisions: Dept. of Biochemistry
Food Protectants and Infestation Control
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Feb 2018 04:11
Last Modified: 14 Feb 2018 04:11
URI: http://ir.cftri.com/id/eprint/13405

Actions (login required)

View Item View Item