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Gold nanoparticle synthesis coupled to fluorescence turn-on for sensitive detection of formaldehyde using formaldehyde dehydrogenase.

Akshath, U. S. and Praveena, Bhatt (2016) Gold nanoparticle synthesis coupled to fluorescence turn-on for sensitive detection of formaldehyde using formaldehyde dehydrogenase. RSC Advances, 6. pp. 54777-54784.

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Abstract

Ultrasensitive detection of toxins by nanoparticle-fluorophore interaction has been studied extensively in the past decade. Present study highlights the detection of formaldehyde based on fluorescence turn-on induced by NADH mediated GNP synthesis. The growth solution consisted of CTAB, Au3+ and fluorescein dye. Extensive fluorescence quenching was observed upon interaction of fluorescein with the growth solution. However, addition of NADH led to a dose dependent fluorescence turn-on. This behavior was successfully employed for ultrasensitive detection of formaldehyde (0.01pg - 300pg/mL with R2=0.9439) as a function of NADH using formaldehyde dehydrogenase enzyme. Metal ion interactions with the proposed system were also studied. We propose that the initial fluorescence quenching is due to CTAB-fluorescein-Au3+ interaction. Addition of NADH to the growth solution led to initiation of gold nanoparticle synthesis by the reduction of Au3+ to Au0 that induced more polarity on the CTAB micellar surface causing fluorescein-CTAB transition and turn-on fluorescence further leading to detection of NADH. Also, at increased NADH/formaldehyde concentration, fluorescence decrease was observed which is due to efficient synthesis of gold nanoparticle leading to spectral overlap. The proposed method of Au3+ reduction coupled to fluorescence turn-on can be applied for monitoring NADH dependent dehydrogenase reactions at ultrasensitive levels.

Item Type: Article
Uncontrolled Keywords: Fluorescence turn-on, gold nanoparticles, Nicotinamide adenine dinucleotide hydrate detection, formaldehyde
Subjects: 600 Technology > 01 Medical sciences > 17 Toxicology
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Jan 2018 06:15
Last Modified: 22 Jan 2018 06:15
URI: http://ir.cftri.com/id/eprint/13361

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