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Unravelling a stearidonic acidrich triacylglycerol biosynthetic pathway in the developing seeds of Buglossoides arvensis: A transcriptomic landscape.

Sreedhar, R. V. and Prasad, P. and Prasanna Anjaneya Reddy, Lebaka and Ram, Rajasekharan and Malathi, Srinivasan (2017) Unravelling a stearidonic acidrich triacylglycerol biosynthetic pathway in the developing seeds of Buglossoides arvensis: A transcriptomic landscape. Scientific Reports, 7. pp. 1-16.

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Abstract

potential applications in human health and nutrition. The molecular basis of SDA biosynthesis in this plant remains unknown due to lack of genomic information. To unravel key genes involved in SDA-rich triacylglycerol (TAG) biosynthesis, we performed transcriptome sequencing of pooled mRNA from five different developmental stages of B. arvensis seeds using Illumina NextSeq platform. De novo transcriptome assembly generated 102,888 clustered transcripts from 39.83 million high-quality reads. Of these, 62.1% and 55.54% of transcripts were functionally annotated using Uniprot-Viridiplantae and KOG databases, respectively. A total of 10,021 SSR-containing sequences were identified using the MISA tool. Deep mining of transcriptome assembly using in silico tools led to the identification of genes involved in fatty acid and TAG biosynthesis. Expression profiling of 17 key transcripts involved in fatty acid desaturation and TAG biosynthesis showed expression patterns specific to the development stage that positively correlated with polyunsaturated fatty acid accumulation in the developing seeds. This first comprehensive transcriptome analysis provides the basis for future research on understanding molecular mechanisms of SDA-rich TAG accumulation in B. arvensis and aids in biotechnological production of SDA in other oilseed crops.

Item Type: Article
Uncontrolled Keywords: Buglossoides arvensis, oilseed crop, stearidonic acid (SDA)
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 18 Jan 2018 08:57
Last Modified: 18 Jan 2018 08:57
URI: http://ir.cftri.com/id/eprint/13316

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