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Molecular Analyses of Vanilla planifolia Cultivated in India using RAPD and ISSR Markers.

Sreedhar, R. V. and Venkatachalam, L. and Roohie, K. and Bhagyalakshmi, N. (2007) Molecular Analyses of Vanilla planifolia Cultivated in India using RAPD and ISSR Markers. Orchid Science and Biotechnology, 1 (1). pp. 29-33.

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The natural “vanilla flavour” obtained from the pods of Vanilla planifolia is the largest flavouring ingredient in food industry. In order to evaluate the usefulness of genetic markers such as RAPD and ISSR for assessing the diversity among clones of V. planifolia and to create a database for the available germplasm, we investigated the existing population cultivated in India. The genetic diversity among 25 accessions collected from 13 major locations was studied. Forty random amplified polymorphic DNA (RAPD) and 11 inter-simple sequence repeats (ISSR) primers resulted in 326 scorable bands ranging in size from 200 bp to 2800 bp and 83 scorable bands from 200 bp to 2500 bp, respectively. Banding pattern among the different samples collected within accessions was similar indicating that the morphological difference observed within accession had no genetic background. On the other hand, molecular analysis among different accessions from different locations also yielded identical PCR band profiles in both RAPD and ISSR analysis. These results clearly indicate that V. planifolia cultivated in India is likely to share the same genetic background and therefore, the genetic diversity is extremely low. Therefore we propose other biotechnological approaches to induce genetic variations to improve the agronomical traits.

Item Type: Article
Uncontrolled Keywords: clonal propagation, DNA polymorphism, genetic diversity, orchid, vanilla flavour
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Dec 2017 09:43
Last Modified: 26 Dec 2017 09:43
URI: http://ir.cftri.com/id/eprint/13236

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