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Cardiolipin deficiency causes triacylglycerol accumulation in Saccharomyces cerevisiae.

Pradeep Kumar, Yadav and Ram, Rajasekharan (2017) Cardiolipin deficiency causes triacylglycerol accumulation in Saccharomyces cerevisiae. Molecular and Cellular Biochemistry, 434. pp. 89-103. ISSN 0300-8177

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Abstract

In yeast, the synthesis of cardiolipin (CL) and phosphatidylethanolamine (PE) occurs mainly in mitochondria. CL and PE have overlapping functions, and they are required for mitochondrial function. PE is physiologically linked with triacylglycerol (TAG) metabolism in Saccharomyces cerevisiae, involving an acyl-CoA-independent pathway through the phospholipid:diacylglycerol acyltransferase activity of the Lro1 protein. There is no report on the physiological link between CL and TAG metabolism. Here we report a metabolic link between CL and TAG accumulation in the S. cerevisiae. Our data indicated that CL deficiency causes TAG accumulation, involving an acyl-CoA-dependent pathway through the diacylglycerol acyltransferase activity of the Dga1 protein with no changes in the TAG molecular species. The DGA1 gene deletion from the CL-deficient strains reduced the TAG levels. Data from in vitro and in vivo analyses showed that CL did not affect the enzymatic activity of Dga1. Our data also showed that CL deficiency leads to the up-regulation of acetyl-CoA synthetase genes (ACS1 and ACS2) of the cytosolic pyruvate dehydrogenase bypass pathway. This study establishes a physiological link between CL and TAG metabolism in S. cerevisiae.

Item Type: Article
Uncontrolled Keywords: Cardiolipin � Triacylglycerol � Lipid droplets � Diacylglycerol acyltransferase � DDHD domain-containing lipase 1 � Metabolic link
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 22 Lipid Chemistry
500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast
Divisions: Dept. of Lipid Science
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Nov 2017 05:26
Last Modified: 21 Nov 2017 05:26
URI: http://ir.cftri.com/id/eprint/13136

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