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Zerumbone Ameliorates High Glucose-Induced Reduction in AMPActivated Protein Kinase Phosphorylation in Tubular Kidney Cells.

Shrikanth, C. B. and Nandini, C. D. (2017) Zerumbone Ameliorates High Glucose-Induced Reduction in AMPActivated Protein Kinase Phosphorylation in Tubular Kidney Cells. Journal of Agricultural and Food Chemistry, 65. pp. 9208-9216. ISSN 0007-1145

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Abstract

AMP-activated protein kinase (AMPK) plays an important role in pathophysiology of diabetes and its complications. In recent years, its role in kidney as a therapeutic target in ameliorating diabetic kidney damage is receiving renewed attention. Efforts on identifying AMPK modulators from dietary sources have gained prominence because of the tremendous potential it harbors. We therefore, examined the effect of a few bioactives on AMPK phosphorylation in kidney tubular cells. AMPK phosphorylation at Thr172 was reduced (0.42 ± 0.05-fold change compared to the control; p < 0.01 vs control) after treatment with high glucose (30 mM) for 48 h and restored by zerumbone (1.59 ± 0.20; p < 0.01 vs high glucose) but not by other tested modulators. Zerumbone also increased the phosphorylation of downstream target of AMPK, the acetyl- CoA carboxylase (ACC) without affecting the mitochondrial membrane potential and ADP/ATP ratio. Thus, zerumbone could potentially be explored as a therapeutic agent in bringing about energy homeostasis in diabetes where high glucose suppresses the AMPK pathway.

Item Type: Article
Uncontrolled Keywords: AMP-activated protein kinase, hyperglycaemia, AMPK activators, zerumbone, MDCK cells
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 16 Protein Biochemistry
600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions: Molecular Nutrition
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 21 Nov 2017 04:18
Last Modified: 21 Nov 2017 04:18
URI: http://ir.cftri.com/id/eprint/13131

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