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Methyl jasmonate modulated biotransformation of phenylpropanoids to vanillin related metabolites using Capsicum frutescens root cultures

Suresh, B. and Ravishankar, G. A. (2005) Methyl jasmonate modulated biotransformation of phenylpropanoids to vanillin related metabolites using Capsicum frutescens root cultures. Plant Physiology and Biochemistry, 43. pp. 125-131.

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Abstract

Normal root cultures of Capsicum frutescens biotransform externally fed precursors, like caffeic acid and veratraldehyde, to vanillin and other related metabolites. The bioconversion of caffeic acid to further metabolites—viz. vanillin, vanillylamine, vanillic acid—was shown to be elicited by treating the cultures with 10 µM methyl jasmonate (MJ). Root cultures treated with MJ accumulated (1.93 times) more of vanillin (20.2 µM on day-3) than untreated ones.A concomitant increase in enzymatic activity of caffeic acid O-methyl transferase (CAOMT, EC 2.1.1.68) was obtained in MJ treated cultures, compared to untreated cultures. After 24 h ofMJ treatment, a 13.7-fold increase in CAOMT activity was recorded in root cultures of C. frutescens. Cultures treated with veratraldehyde accumulated more vanillin (78 µM) than caffeic acid fed cultures, 6 days after precursor addition. Capsaicin did not accumulate even after addition of precursors. The efficiencies of biotransformation with caffeic acid and veratraldehyde were 2.2% and 9% with respect to vanillin formation, indicating a possible diversion of the phenylpropanoid pathway towards other secondary metabolites.

Item Type: Article
Uncontrolled Keywords: Capsicum frutescens; Normal roots; Biotransformation; Caffeic acid; Veratraldehyde; Methyl jasmonate; Caffeic acid O-methyl transferase
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 04 Jul 2007 11:19
Last Modified: 28 Dec 2011 09:28
URI: http://ir.cftri.com/id/eprint/1294

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