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Enzymatic esterification of free fatty acids of hydrolyzed soy deodorizer distillate in supercritical carbon dioxide

Nagesha, G. K. and Manohar, B. and Udaya Sankar, K. (2004) Enzymatic esterification of free fatty acids of hydrolyzed soy deodorizer distillate in supercritical carbon dioxide. Journal of Supercritical Fluids, 32. pp. 137-145.

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Abstract

Supercritical carbon dioxide (SC-CO2) as a medium for esterification of free fatty acids (FFA) of hydrolyzed soy deodorizer distillate (DOD) with butanol, has been studied using Mucor miehei lipase as a biocatalyst. Process conditions (pressure, temperature, butanol and enzyme concentration and incubation time) were optimized by conducting experiments at five different levels using a central composite rotatable design (CCRD) method. A second order polynomial response surface equation was developed indicating the effect of variables on ester yield. Contour maps generated using the response surface equation, showed that all the experimental variables significantly affected the yield of fatty acid butyl esters (FABE). The reaction variables influenced the yield of esters both independently and in combination. A pressure of 122 bar, temperature 36 ◦C, butanol concentration 1.2 M, enzyme concentration 15% (w/w) and incubation time of 3 h were predicted to be the optimal for the maximum yield of 100% FABE, which was validated by an experiment to yield 95% of FABE. SC-CO2 is a potential medium for the biomodification of soy DOD by esterification of free fatty acids with butanol.

Item Type: Article
Uncontrolled Keywords: Hydrolysed soy deodorizer distillate (DOD); Free fatty acids (FFA); Esterification; Mucor miehei lipase; Supercritical carbon dioxide (SC-CO2); Fatty acid butyl esters (FABE)
Subjects: 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Jun 2007 12:10
Last Modified: 28 Dec 2016 11:09
URI: http://ir.cftri.com/id/eprint/1278

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