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Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship.

Sabeeta, Kapoor and Shylaja, M. Dharmesh (2017) Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship. Carbohydrate Polymers, 160. pp. 52-61.

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Abstract

tPectic Polysaccharide (PP) from dietary sources has been known to prevent cancer growth and henceimpede cancer progression. We evaluated anticancer effect of Pectic-Oligosaccharide isolated from SourRaw Tomato (SrTPO); its bioavailability and structure elucidated from purified fraction (SrTPO1). SrTPO1inhibited galectin-3 activity with MIC of 0.25 ug/mL (100 fold better than standard galactose), inhibitedthe growth of AGS cells (IC503.4 �g/mL) and induced apoptosis (70% inhibition at 30 �g/mL concentra-tion). Normal– NIH 3T3 cells were not affected by SrTPO as opposed to doxorubicin, a known anticancerdrug, which reduced 76% viability at equivalent dose. SrTPO1 was identified as RhamnogalacturonanI-arabinogalactan (RGI-AG), where repeated alternative rhamnose and galacturonic acid residues wereobserved while arabinose in the branch point and �-1,4 linked galactose in the linear chain form. SrTPOwas found to be bioavailable as evaluated by FITC labelled oligos inside the cell, which was in reciprocalproportion with apoptosis.

Item Type: Article
Uncontrolled Keywords: Sour raw tomato Pectic polysaccharide Pectic-oligosaccharide Rahmnogalactouronanl-arabinogalactan Gastric cancer Bioavailability
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
600 Technology > 08 Food technology > 23 Vegetables > 03 Tomato
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 10 Jul 2017 08:35
Last Modified: 10 Jul 2017 08:35
URI: http://ir.cftri.com/id/eprint/12777

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