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In vitro clonal propagation of bird eye chilli (Capsicum frutescens Mill.)

Gururaj, H. B. and Giridhar, P. and Ashwani, Sharma and Prasad, B. C. N. and Ravishankar, G. A. (2004) In vitro clonal propagation of bird eye chilli (Capsicum frutescens Mill.). Indian Journal of Experimental Biology, 42. pp. 1136-1140.

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An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skooy (MS) medium containing 0.5-3.0 mgr1 of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg r of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gr1 activated charcoal (AC) were used for shoot regeneration from both shoot tip and nodal explants. Shoot tip explants (100%) grew well on medium containing 1 mgr' of kinetin and 1 mgl-l ofIBA. Shoot proliferation (1-3) from nodal explants was effective on this medium. The regenerated shoots with 4-7 nodes had further growth upon sub-culturing onto kinetin (1 mgr1 and IBA (1 mgr1) and rooted simultaneously. The rooted plants were transferred to pots after hardening under controlled conditions. The survival percentage in pots was 80-90%.

Item Type: Article
Uncontrolled Keywords: Bird eye chilli, Capsicumfrutescens, Nodal explant, Plantlet, Regeneration, Rooting, Shoot tip
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Jun 2007 11:51
Last Modified: 30 Jun 2015 06:02
URI: http://ir.cftri.com/id/eprint/1275

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