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Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence.

Vemana, Gowd and Sandeep, M. S. and Nandini, C. D. (2016) Effect of pathological conditions on peritoneal macrophage glycosaminoglycans: Impact on cytoadherence. International Journal of Biological Macromolecules, 92. pp. 1183-1190. ISSN 0141-8130

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tGlycosaminoglycans (GAGs) have a plethora of functions to play. They are widely present in extracellularmatrix, cell surface and inside the cell. During pathological conditions remodeling of GAGs leads to mod-ifications in their structure and functions. In the present work, peritoneal macrophages were isolatedfrom normal, diabetic, and diet-induced hypercholesterolemic rats and evaluated in terms of GAGs andcytoadherence to various extracellular matrix (ECM) components. Peritoneal macrophages are known toplay important roles in the control of infection and inflammation. Isolated GAGs were characterized asbelonging to heparan sulfate/heparin class. There were quantitative changes in sulfated GAGs in diabeticand hypercholesterolemic groups when compared to normal rats. Dose-dependent changes in cytoadher-ence were observed only with respect to fibronectin in LPS-activated macrophages from diabetic animalsbut not with laminin and type IV collagen when compared to macrophages from normal rats. Cytoad-herence was significantly decreased on treatment with heparinase indicating that cytoadherence was atleast partly mediated by heparan sulfate/heparin class of GAGs. Global disaccharide composition analysisshowed that GAGs from macrophages of diabetic animals had higher sulfation ratio when compared tothat of control and hypercholesterolemic animals.

Item Type: Article
Uncontrolled Keywords: Peritoneal macrophages Glycosaminoglycans Heparin/Heparan sulfate Cytoadherence
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 02 Amino Acid Biochemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Jun 2017 11:05
Last Modified: 30 Jun 2017 11:05
URI: http://ir.cftri.com/id/eprint/12732

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