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A novel beta-carotene–associated carrot (Daucus carota L.) pectic polysaccharide.

Sindhuja, H. N. and Marjan, A. and Srinivas, P. and Shylaja, M. Dharmesh (2016) A novel beta-carotene–associated carrot (Daucus carota L.) pectic polysaccharide. Nutrition, 32. pp. 818-826.

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Abstract

Objective: Carrot (Daucus carota L.) is a nutritional source with enriched b-carotene (0.01%) and pectins (0.8%). Although studies have highlighted the association between b-carotene and carrot pectic polysaccharide (CRPP), the precise binding of b-carotene to CRPP and its biological implications were not yet clearly understood. The aim of the present study is to report for the first time a natural carbohydrate polymerdCRPPdwith associated b-carotene. Methods: The presence of b-carotene in CRPP and its binding is demonstrated by various studies including differential extraction followed by liquid chromatography (LC), liquid chromatography mass spectrometry (LC MS), and Fourier transform infrared spectroscopy (FTIR). Further, we show the binding via its release from CRPP using LC, fermentation studies and scanning electron microscopy (SEM) studies. The role of b-carotene in induction of apoptosis and the galactic-3 inhibitory property was also studied. Results: The presence of b-carotene in CRPP was demonstrated, as well as an association between b-carotene and CRPP, apoptotic and galectin-3 inhibitory properties of CRPP, and upregulation of connexin 43 by CRPP. Conclusion: The data highlights that with the combination of b-carotene with CRPP, the prooxidant effect of b-carotene may be reduced, an apoptotic effect may be enhanced, and the anticancer potential of CRPP thus may be strengthened via galectin-3 inhibition.

Item Type: Article
Uncontrolled Keywords: Daucus carota L. Pectic polysaccharide b-carotene Bioavailability HPLC Connexin 43
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
600 Technology > 08 Food technology > 23 Vegetables > 04 Carrot
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Jun 2017 09:25
Last Modified: 30 Jun 2017 09:25
URI: http://ir.cftri.com/id/eprint/12726

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