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Detection and Removal of Sample Matrix Effects in Agrochemical Immunoassays

John H, Skerritt and Amita Rani, B. E. (1996) Detection and Removal of Sample Matrix Effects in Agrochemical Immunoassays. In: Immunoassays for Residue Analysis. American Chemical Society, pp. 29-43.

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Abstract

With the completion of the initial development of assays for many of the most important agrochemicals, there has been an increased focus on actual applications of agrochemical immunoassays to food and environmental matrices. A major prerequisite to assay utilization has been the need to identify and remove matrix interferences, which may result in I residue-free samples appearing positive, or else samples containing residues appearing negative; or 2. changes in the sensitivity of residue detection, from either or both shins in the assay absorbance values in the absence of pesticide or in the standard curve, Matrix: effects are best detected by analysis of spikes of pesticide standards into a solvent extract of pesticide-free matrix and comparison of this concentration response curve with that obtained using standards prepared in solvent alone. A number of approaches to the removal of matrix interference is reviewed, with particular reference to examples from the analyses of insecticide residues in plant-derived foods, including grain and fruit products.

Item Type: Book Section
Uncontrolled Keywords: agrochchemical immunoassays matrix interferences
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
Divisions: Food Protectants and Infestation Control
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 03 Jan 2017 12:41
Last Modified: 03 Jan 2017 12:41
URI: http://ir.cftri.com/id/eprint/12603

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