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Purification of a Class B1 platelet aggregation inhibitor phospholipase A2 from Indian cobra (Naja Naja) venom

Satish, S. and Tejaswini, J. and Krishnakantha, T. P. and Veerabasappa Gowda, T. (2004) Purification of a Class B1 platelet aggregation inhibitor phospholipase A2 from Indian cobra (Naja Naja) venom. Biochimie, 86. pp. 203-210.

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Abstract

A platelet aggregation inhibitor phospholipase A2 (NND-IV-PLA2) was isolated from Naja naja (Eastern India) venom by a combination of cation and anion exchange chromatography. NND-IV-PLA2 is the most catalytically active enzyme isolated from the Indian cobra venom. The acidic PLA2 profile of Eastern regional Indian cobra venom is distinctly different from that of the western regional venom. However the acidic PLA2s from both the regions follow the pattern of increasing catalytic activity with increase in acidic nature of the PLA2 isoform. NND-IV-PLA2 is a Class B1 platelet aggregation inhibitor and inhibits platelet aggregation induced by ADP, collagen and epinephrine. Modification of active site histidine abolishes both catalytic activity and platelet aggregation inhibition activities while aristolochic acid, a phospholipase A2 inhibitor has only partial effect on the two activities.

Item Type: Article
Uncontrolled Keywords: Class B1 Platelet aggregation inhibitor; Phospholipase A2; Aristolochic acid; Naja naja
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Jun 2007 10:48
Last Modified: 14 Dec 2016 11:30
URI: http://ir.cftri.com/id/eprint/1253

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