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Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility

Rajesh Kumar, T. and Doreswamy, K. and Shrilatha, B. and Dr., Muralidhara (2002) Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility. Mutation Research, 513. pp. 103-111.

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Abstract

Our previous work has shown that prooxidant treatment has the propensity to induce male-mediated dominant lethal (DL) type mutations in mice. The present investigation is aimed to understand the effect of oxidative stress (OS) on DNA damage in testis, epididymal sperms and its propensity to induce sperm head abnormalities as well as its implications on male fertility in mice. Initially, employing two organic hydroperoxides, (t-butyl hydroperoxide, t-bHP and cumene hydroperoxide, cHP) as model prooxidants, induction of oxidative stress was ascertained following single/multiple sublethal doses. Further, the multiple exposure modelwas utilized to characterize effects on testicular weights, histoarchitecture, caudal sperm counts, lipid peroxidation, DNA damage and frequency of abnormal sperms. Single sublethal doses (1/20, 1/10 and 1/5 LD50) of t-bHP and cHP administered (i.p.) to adult mice resulted in only a marginal increase (20% at the highest dosage) in testicular MDA levels. However, multiple doses (1/10 and 1/5 LD50 per day for 5 days) induced marked OS in testis and epididymal sperms as evidenced by amarked increase in lipid peroxidation at 24 h after the last dose. This was associated with significant increase in the DNAdamage (FADUassay) in the testicular tissue. While caudal sperm counts determined at all sampling weeks showed no treatment related alterations, analysis for head abnormalities revealed nearly 2–3-fold increase in the percent abnormal sperms among the hydroperoxide treated mice during the first 3 weeks. Furthermore, mating of prooxidant treated males sequentially for a period of 5 weeks with untreated females resulted in a significant reduction in average pup number per litter during the first 3 weeks. These results suggest that oxidative stress in testicular milieu is associated with DNA damage and produces higher frequency of abnormal sperms with significant effect on male fertility.

Item Type: Article
Uncontrolled Keywords: Organic hydroperoxides; Oxidative stress; Testis; Lipid peroxidation; DNA damage; Abnormal sperms; Male fertility
Subjects: 600 Technology > 01 Medical sciences > 09 Human Physiology
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Jun 2007 10:09
Last Modified: 28 Dec 2016 08:31
URI: http://ir.cftri.com/id/eprint/1250

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