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Rapid Clonal Propagation and Stevioside Profiles of Stevia rebaudiana Bertoni.

Giridhar, P. and Sowmya, K. S. and Ramakrishna, A. and Ravishankar, G. A. (2010) Rapid Clonal Propagation and Stevioside Profiles of Stevia rebaudiana Bertoni. International Journal of Plant Developmental Biology, 4 (1). pp. 47-52.

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Abstract

Shoot tips of Stevia rebaudiana Bertoni were cultured on Murashige and Skoog (MS) or B5 medium, supplemented with 6-benzyladenine (BA), indole-3-butyric acid (IBA) or D-naphthalene acetic acid (NAA) in different combinations. A maximum of 28 shoots per shoot tip explant were produced on B5 medium supplemented with 4.44 μM BA and 0.80 μM NAA. Significantly more shoots could be obtained from in vitro shoot tips of Stevia by repeating the cycle. Elongation of microshoots was significant on B5 medium devoid of plant growth regulators. MS medium containing 0.05 μM-0.25 μM gibberellic acid (GA3) led to elongation of shoots up to 10-12 cm in length with 7-8 nodes each. In vitro leaves derived from primary shoots could regenerate 3-4 shoots on the same medium. In vitro rooting of microshoots (~4 cm in length) was efficient on MS basal medium with shoots 12-13 cm long having 5 nodes and 11-12 roots in one month. After hardening in micropots for one month 80% of the rooted plants survived. In another study, incorporation of 13.62 μM thidiazuron (TDZ) in modified MS medium induced 11-12 multiple shoots from shoot tip explants when inoculated in reverse polarity i.e. shoot tips in downward direction (inverted mode). Both these methods could be useful for mass multiplication of S. rebaudiana. The stevioside content of both in vitro leaves and hardened three-months-old ex vitro plants was analysed by HPLC. The highest stevioside content (6.72% steviolbioside and 0.11% rebaudioside-A on a dry weight basis) were found in ex vitro and in vitro leaves, respectively.

Item Type: Article
Uncontrolled Keywords: elongation, in vitro rooting, micropropagation, shoot tip, steviosides
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Nov 2016 12:10
Last Modified: 16 Feb 2018 04:51
URI: http://ir.cftri.com/id/eprint/12428

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