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Inhibition of LDL oxidation and oxidized LDLinduced foam cell formation in RAW 264.7 cells show antiatherogenic properties of a foliar methanol extract of Scoparia dulcis

Sinjitha, S. Nambiar and Nandini, P. Shetty and Praveena, Bhatt and Bhagyalakshmi, N. (2014) Inhibition of LDL oxidation and oxidized LDLinduced foam cell formation in RAW 264.7 cells show antiatherogenic properties of a foliar methanol extract of Scoparia dulcis. Pharmacognosy Magazine, 10 (2). S240-S248.

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Abstract

Background: Oxidation of low density lipoproteins and their further uptake by macrophages is known to result in the formation of foam cells, which are critical in the initiation of atherosclerosis through activation of inflammatory signalling cascades. Thus, powerful dietary antioxidants are receiving attention for the reversal of such pathological states. Materials and Methods: Extracts of Scoparia dulcis have been used as tea and health drinks with various health promoting effects. In the present study, we examined the reactive oxygen scavenging potential as well as antiinflammatory and antiatherogenic efficacies, using leaf extracts obtained after successive extraction with various solvents. Results: A methanol extract showed potent antioxidant activity with an IC value of 570 μg/ml, caused hydrogen peroxide scavenging (28.9 μg/ml) and antiinflammatory effects by improving human erythrocyte membrane stabilisation (about 86%). The methanol extract also efficiently inhibited lipid peroxidation and oxidation of low density lipoproteins, thus preventing foam cell formation in cultured RAW 264.7 cells. Furthermore, phytochemical screening of the extracts showed high accumulation of flavonoids. Conclusions: The foliar methanol extract of Scoparia dulcis has a strong antiatherogenic potential and this property could be attributed maybe due to presence of flavonoids since HPLC analysis showed high concentrations of myricetin and rutin in the methanol extract.

Item Type: Article
Uncontrolled Keywords: Antiatherogenic, antiinflammatory, flavonoids, foam cells, Scoparia dulcis
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 18 Flavonoid Chemistry
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Nov 2016 06:26
Last Modified: 07 Nov 2016 06:26
URI: http://ir.cftri.com/id/eprint/12390

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