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Quantum dots as optical labels for ultrasensitive detection of polyphenols

Akshath, U. S. and Shubha, L. and Praveena, Bhatt and Thakur, M. S. (2014) Quantum dots as optical labels for ultrasensitive detection of polyphenols. Biosensors and Bioelectronics, 57. pp. 317-323.

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Abstract

Considering the fact that polyphenols have versatile activity in-vivo, its detection and quantification is very much important for a healthy diet. Laccase enzyme can convert polyphenols to yield mono/polyquinones which can quench Quantum dots fluorescence. This phenomenon of charge transfer from quinones to QDs was exploited as optical labels to detect polyphenols. CdTe QD may undergo dipolar interaction with quinones as a result of broad spectral absorption due to multiple excitonic states resulting from quantum confinement effects. Thus, “turn-off” fluorescence method was applied for ultrasensitive detection of polyphenols by using laccase. We observed proportionate quenching of QDs fluorescence with respect to polyphenol concentration in the range of 100 µg to 1 ng/mL. Also, quenching of the photoluminescence was highly efficient and stable and could detect individual and total polyphenols with high sensitivity (LOD-1 ng/mL). Moreover, proposed method was highly efficient than any other reported methods in terms of sensitivity, specificity and selectivity. Therefore, a novel optical sensor was developed for the detection of polyphenols at a sensitive level based on the charge transfer mechanism.

Item Type: Article
Uncontrolled Keywords: Quantum dots; Polyphenols; Electron transfer; Laccase
Subjects: 600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Sep 2016 12:44
Last Modified: 22 Sep 2016 12:44
URI: http://ir.cftri.com/id/eprint/12315

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