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Studies on A Maltohexaose (G6) Producing Alkaline Amylase from A Novel Alkalophilic Streptomyces Species

Geetha, Subramanian and Saravanan, Ayyadurai and Trupti, Sharma and Sridevi Annapurna, Singh (2012) Studies on A Maltohexaose (G6) Producing Alkaline Amylase from A Novel Alkalophilic Streptomyces Species. IIOABJ Journal, 3 (3). pp. 15-30.

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The present paper describes the production and characterization of alkaline amylases from a novel obligate alkalophilic Streptomyces sp. This strain was isolated from the alkaline soil of the meteorite Lonar lake, situated in Maharashtra, India. This is the first report of a maltohexaose producing alkaline amylase from an alkalophilic Streptomyces sp and the fourth report of an alkalophilic Streptomyces sp producing an alkaline amylase. The optimum growth of the culture occurred in the range of pH 8 to 10 at 28o C. No visible growth occurred at neutral or acidic pH. Optimum enzyme production occurred only at alkaline pH, the optimum being pH 10.5. Soluble starch as well as raw starch induced the enzyme, induction being higher with raw starch. Substantial quantities of the enzyme was also produced in a non- starch medium containing malt extract, yeast extract and glucose. The enzyme was active in the range of pH 7-10, the optimum pH being 9.0 at 45oC. Starch electrophoresis of the culture filtrate revealed the presence of two amylolytic bands. The molecular weights of the two bands as determined by native electrophoresis were 36,920 and 36,300 daltons respectively. The enzyme was found to be endo in action, the predominant products being maltohexaose (48%) followed by maltotetrose, maltotriose and maltose, at the initial stages of hydrolysis as well as after 24 hours and hence would have a potential application in the food and pharmaceutical industry. The enzyme preparation was also effective in removing starch based stains. Hence, the alkaline amylases from the Streptomyces sp would have a potential application in the food and detergent industries.

Item Type: Article
Uncontrolled Keywords: Alkalophilic Streptomyces sp; alkaline amylase; maltohexaose; application in food and detergent industries
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Sep 2016 05:51
Last Modified: 08 Sep 2016 05:51
URI: http://ir.cftri.com/id/eprint/12277

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