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Beneficial Effect of a Probiotic Lactobacillus Fermentum Cfr 2195 in Trinitrobenzenesulfonate Induced Colitis in Rat

GirishKumar, B. and David, Elisha Henry and Krishnapura, Srinivasan and Prapulla, S. G. (2012) Beneficial Effect of a Probiotic Lactobacillus Fermentum Cfr 2195 in Trinitrobenzenesulfonate Induced Colitis in Rat. Annals Food Science and Technology, 13 (2). pp. 231-239.

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Abstract

The intestinal anti-inflammatory effects of L. fermentum CFR 2195, isolated from healthy breast fed infant’s fecal matter was evaluated. Ulcerative colitis was induced in Wistar rats by intracolonic administration of TNBS. L. fermentum CFR 2195 was administered orally (5x108 colony-forming units) to rats daily for 3 weeks, starting 2 weeks prior to the induction of ulcerative colitis. Normal control group of rats were fed solely with basal diet. Probiotic control group was fed with basal diet along with probiotic administration. Among the colitis induced rats, the TNBS control group was fed with basal diet whereas, the TNBS-probiotic group was fed with basal diet along with probiotic administration. Colonic damage was evaluated both biochemically, histologically and the luminal contents were used for the microbiological studies. The intestinal anti-inflammatory effects of L. fermentum CFR 2195 were evidenced by a significant reduction in colonic myeloperoxidase activity (P<0·05) and also in histopathological changes. The results regarding the microbiological analysis revealed that when the lactobacilli: pathogen ratio was evaluated, the inflammatory condition did result in a significant decrease (P<0·05) in comparison with normal control group; the administration of probiotic L. fermentum CFR 2195 resulted in the restoration of the normal balance of lactobacilli: pathogen ratio. Treatment with L. fermentum CFR 2195 also significantly counteracted the colonic glutathione depletion induced due to the oxidative stress caused by the inflammatory process. In conclusion, it can be stated that pretreatment with L. fermentum CFR 2195 has shown anti-inflammatory activity in the intestines of TNBS model of rat colitis.

Item Type: Article
Uncontrolled Keywords: experimental colitis, trinitrobenzenesulfonic acid, L. fermentum CFR 2195, probiotic
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Dept. of Biochemistry
Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 08 Sep 2016 05:40
Last Modified: 19 Dec 2016 13:12
URI: http://ir.cftri.com/id/eprint/12276

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