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Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid

Kavitha, M. D. and Kathiresan, S. and Sila, Bhattacharya and Sarada, R. (2016) Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid. Journal of Food Science and Technology, 53 (5). pp. 2270-2278. ISSN 0022-1155

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Abstract

Porphyridium purpureum a red marine microalga is known for phycobiliproteins (PB), polyunsaturated fatty acids and sulphated exopolysaccharides. In the present study, effects of media constituents for the production of different polyunsaturated fatty acids from P. purpureum were considered using a response surface methodology (RSM). A second order polynomial was used to predict the response functions in terms of the independent variables such as the concentrations of sodium chloride, magnesium sulphate, sodium nitrate and potassium dihydrogen phosphate. The response functions were production of biomass yield, total lipid and polyunsaturated fatty acids like arachidonic acid (AA 20:4) and eicosapentaenoic acid (EPA 20:5). Results corroborated that maximumBiomass (0.95 gL−1) yield was at the concentrations of sodium chloride (14.89 gL−1), magnesium sulfate (3.93 gL−1) and sodium nitrate (0.96 gL−1) and potassium dihydrogen phosphate (0.09 gL−1). Optimum total lipid (17.9 % w/w) and EPA (34.6 % w/ w) content was at the concentrations of sodium chloride (29.98 gL−1), magnesium sulfate (9.34 gL−1) and sodium nitrate (1.86 gL−1). Variation in concentration of potassium dihydrogen phosphate for both lipid (0.01gL−1) and EPA content (0.20 gL−1) was observed. The optimum conditions for biomass, total lipid, AA and EPA varied indicating their batch mode of growth and interaction effect of the salt.

Item Type: Article
Uncontrolled Keywords: Porphyridium . Fatty acids . Response surface methodology (RSM) . Optimization . Eicosapentaenoic acid
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
Divisions: Grain Science and Technology
Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 25 Aug 2016 12:45
Last Modified: 25 Aug 2016 12:45
URI: http://ir.cftri.com/id/eprint/12265

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