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Metabolic engineering of Dunaliella salina for production of ketocarotenoids

Anila, N. and Daris, P. Simon and Arun, Chandrashekar and Ravishankar, G. A. and Sarada, R. (2016) Metabolic engineering of Dunaliella salina for production of ketocarotenoids. Photosynthesis Research, 127. pp. 321-333.

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Abstract

Dunaliella is a commercially important marine alga producing high amount of b-carotene. The use of Dunaliella as a potential transgenic system for the production of recombinant proteins has been recently recognized. The present study reports for the first time the metabolic engineering of carotenoid biosynthesis in Dunaliella salina for ketocarotenoid production. The pathway modification included the introduction of a bkt gene from H. pluvialis encoding b-carotene ketolase (4,40b-oxygenase) along with chloroplast targeting for the production of ketocarotenoids. The bkt under the control of Dunaliella Rubisco smaller subunit promoter along with its transit peptide sequence was introduced into the alga through standardized Agrobacterium-mediated transformation procedure. The selected transformants were confirmed using GFP and GUS expression, PCR and southern blot analysis. A notable upregulation of the endogenous hydroxylase level of transformants was observed where the BKT expression was higher in nutrient-limiting conditions. Carotenoid analysis of the transformants through HPLC and MS analysis showed the presence of astaxanthin and canthaxanthin with maximum content of 3.5 and 1.9 lg/g DW, respectively. The present study reports the feasibility of using D. salina for the production of ketocarotenoids including astaxanthin.

Item Type: Article
Uncontrolled Keywords: Dunaliella Carotenoids Astaxanthin Metabolic engineering
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 10 Aug 2016 12:23
Last Modified: 10 Aug 2016 12:23
URI: http://ir.cftri.com/id/eprint/12246

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