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Effect of licorice extract on cell viability, biofilm formation and exotoxin production by Staphylococcus aureus

Rohinishree, Y. S. and Negi, P. S. (2016) Effect of licorice extract on cell viability, biofilm formation and exotoxin production by Staphylococcus aureus. Journal of Food Science and Technology, 53 (2). pp. 1092-1100.

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Abstract

Staphylococcus aureus is one of the most significant clinical pathogen, as it causes infections to humans and animals. Even though several antibiotics and other treatments have been used to control S. aureus infections and intoxication, bacterium is able to adapt, survive and produces exotoxins. Licorice (Glycyrrhiza glabra L.) has been used traditionally in various medicinal (antimicrobial) preparations, and Glycyrrhizic acid (GA) is the major active constituents present in it. In the present investigation the effect of licorice extract on methicillin susceptible S. aureus (FRI 722) and methicillin resistant S. aureus (ATCC 43300) growth and toxin production was studied. The MIC of licorice extract was found to be 0.25 and 2.5 mg GA ml−1 against S. aureus FRI 722 and S. aureus ATCC 43300, respectively. Inhibition of biofilm formation was observed even at very low concentration (25 μg GA ml−1). Gradual decrease in expression and production of exotoxins such as α and β hemolysins and enterotoxin B was observed with the increasing concentrations of licorice extract, however, suboptimal concentration induced the expression of some of the virulence genes. This study indicated efficacy of licorice extract in controlling growth and pathogenicity of both methicillin susceptible and methicillin resistant S. aureus, however, the mechanisms of survival and toxin production at suboptimal concentration needs further study.

Item Type: Article
Uncontrolled Keywords: Staphylococcus aureus . Glycyrrhizic acid . Biofilm . Western blot . Exotoxins
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
600 Technology > 08 Food technology > 10 Food Microorganisms
Divisions: Fruit and Vegetable Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 May 2016 09:49
Last Modified: 30 May 2016 09:49
URI: http://ir.cftri.com/id/eprint/12221

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