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Studies On Immobilized Enzymes For Biosensor Applications

Devaraja Gouda, M. (2000) Studies On Immobilized Enzymes For Biosensor Applications. PhD thesis, University of Mysore.


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In the context of the usage of immobilized enzymes for sugar biosensor applications, the present investigations attempt to a better understanding of the various aspects of the enzyme applications. The work has involved the construction of an immobilized enzyme based biosensor for glucose and sucrose in food and fermentation analysis, study of the effect of various parameters on the performance of the biosensor and also basic biochemical aspects of the immobilized single and multienzyme systems with respect to the effect of temperature, pH, denaturants as well as operational stability. Aspects considered include: Construction and optimization of biosensor, Estimation of sucrose in the presence of glucose, Stability of single and multienzyme systems, Thermal stability studies of immobilized enzymes using biosensor, Mechanism of thermal inactivation of GOD, Reversible denaturation of the immobilized single and multienzyme systems,

Item Type: Thesis (PhD)
Uncontrolled Keywords: immobilized enzymes biosensor applications
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Jun 2007 09:34
Last Modified: 28 Dec 2011 09:28
URI: http://ir.cftri.com/id/eprint/1215

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