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Plant Cell Cultures for the Production of Phytochemicals - Anthocyanin and Capsaicin in Bioreactors

Madhusudhan, R. (1998) Plant Cell Cultures for the Production of Phytochemicals - Anthocyanin and Capsaicin in Bioreactors. PhD thesis, University of Mysore.

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Abstract

The present investigation dealt with the studies on production of anthocyanin from Daucus carota cell cultures, and capsaicin from immobilized cells of Capsicum frutescens at shake flask, and lab scale reactor level. The specific objectives of the study were as follows: i)To develop a high yielding cell line of Daucus carota. ii)To establish and maintain the cell suspension cultures of D. carota.iii)Optimization of process parameters of the cell culture process for anthocyanin production. The parameters included nutrient medium, hormonal supplementation,temp, pH, light, dissolved oxygen. iv) To study the kinetics of growth and production of anthocyanin by D. carota cells in suspension cultures. v)Comparative evaluation of various methods for estimation of biomass in plant cell suspension cultures.vi)Growth, and production of anthocyanin from D. carota cells in bioreactor at 2 L level.vii)Rheological characterization of the culture broth in bioreactor. viii) Immobilization of Capsicum frutescens cells in alginate, and polyurethane foam matrices. ix)Studies on continuous production of capsaicin from immobilized C. frutescens cells using a suitable column reactor.

Item Type: Thesis (PhD)
Uncontrolled Keywords: Plant cell culture phytochemicals anthocyanins Capsaicin Daucus carota Capsicum frutescens
Subjects: 600 Technology > 08 Food technology > 31 Food Additives
600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
500 Natural Sciences and Mathematics > 10 Plants > 01 Plant Cell
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Jun 2007 09:05
Last Modified: 28 Dec 2011 09:28
URI: http://ir.cftri.com/id/eprint/1214

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