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Antioxidant and neuroprotective potential of chitooligomers inCaenorhabditis elegans exposed to Monocrotophos.

Nidheesh, T. and Chinnu, Salim and Rajini, P. S. and Suresh, P. V. (2016) Antioxidant and neuroprotective potential of chitooligomers inCaenorhabditis elegans exposed to Monocrotophos. Carbohydrate Polymers, 135 (1). pp. 138-144.

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tThe objectives of this investigation were to establish the propensity of the chitooligomers (COS) to amelio-rate neurodegeneration and oxidative stress in Caenorhabditis elegans induced by an organophosphorusinsecticide, Monocrotophos (MCP). COS was prepared from �-chitosan by the enzymatic method usingchitosanase and characterized by HPLC and electron spray ionization-TOF-(ESI-TOF)-MS. We exposedage synchronized L4 C. elegans worms (both wild type N2 and transgenic strain BZ555 (Pdat-1:GFP) tosublethal concentration of MCP (0.75 mM) for 24 h in the presence or absence of COS (0.2 mM). Theneuroprotective effect of COS was examined in N2 worms in terms of brood size, lifespan, egg laying,dopamine content, acetylcholinesterase and carboxylesterase activity and by direct visualization andquantification of degeneration of dopaminergic neurons in BZ555. Exposure to COS extended lifespan,normalized egg laying, increased brood size, decreased the dopaminergic neurodegeneration, increasedthe dopamine content and increased AChE and carboxylesterase activity in C. elegans treated with MCP.COS induced a significant decrease in reactive oxygen species and increased the reduced glutathione levelas well as increased superoxide dismutase and catalase activity. Our findings demonstrate that COS sig-nificantly inhibits the dopaminergic neurodegeneration and associated physiological alterations inducedby MCP in C. elegans by attenuating the oxidative stress as well.

Item Type: Article
Uncontrolled Keywords: Chitooligomers Monocrotophos Caenorhabditis elegans Antioxidant Oxidative stress Neuroprotection
Subjects: 600 Technology > 01 Medical sciences > 17 Toxicology
600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Food Protectants and Infestation Control
Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Jan 2016 08:12
Last Modified: 28 Nov 2016 08:00
URI: http://ir.cftri.com/id/eprint/12084

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