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Role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression, and induction of apoptosis in HeLa cells.

Sowmya, Poorigali Raghavendra and Arathi, Bangalore Prabhashankar and Vijay, K. and Baskaran, V. and Lakshminarayana, Rangaswamy (2015) Role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression, and induction of apoptosis in HeLa cells. Molecular and Cellular Biochemistry, 406 (1). pp. 245-253.

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Abstract

The objective of the present study was to determine the role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression and induction of apoptosis in HeLa cells. Cells (5 9 103) were treated with different concentrations (25–100 lM) of b-carotene (BC) or lutein (L) or astaxanthin (AST) dissolved in 0.5 % of tetrahydrofuran (THF), dimethylsulfoxide (DMSO), and fetal bovine serum (FBS), respectively. The effect of delivery vehicle on carotenoids uptake, cytotoxicity, oxidative status, cell cycle distribution, and apoptosis was examined after 48 h of incubation. The results shown that, cell viability reduced significantly in a dose- and time-dependent manner irrespective of carotenoid delivered in vehicles. Cellular uptake of BC delivered in THF was higher by 49.1, 29.7 % and L delivered through THF was higher by 41.7 and 37.5 % than DMSO and FBS, respectively. While, AST delivered through DMSO was higher by 36.1 and 43.7 % than the THF and FBS, respectively. In case of cells treated either with BC or L delivered through THF and AST in DMSO decreased the glutathione and increased the malondialdehyde levels. The net increase in the G2/M phase percentage of cell cycle progression was observed in carotenoid-treated cells. The % induction of apoptosis by BC or L delivered with THF and AST in DMSO was higher than other treated groups. In conclusion, choice of suitable vehicle for specific carotenoids delivery is essential that in turn may influence on cell proliferation and cell-based assays.

Item Type: Article
Uncontrolled Keywords: Carotenoids Cytotoxicity Cell cycle Apoptosis HeLa cells
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Dec 2015 08:25
Last Modified: 11 Dec 2015 08:25
URI: http://ir.cftri.com/id/eprint/12031

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