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Enhancement of Carotenoids in Green Alga-Botryococcus braunii in Various Autotrophic Media under Stress Conditions.

Ranga Rao, A. and Sarada, R. and Ravishankar, G. A. (2010) Enhancement of Carotenoids in Green Alga-Botryococcus braunii in Various Autotrophic Media under Stress Conditions. International Journal of Biomedical and Pharmaceutical Sciences, 4 (2). pp. 87-92.

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Abstract

Botryococcus braunii is a green colonial micro alga that is used mainly for the production of hydrocarbons, exopolysaccharides, and carotenoids. Growth and carotenoid production of B. braunii culture were studied by using autotrophic media such as CHU13, Z8, BBM and BG11 under stress conditions at high light intensity (3.5 ± 0.2 klux). The cultures were grown under low light (1.5 ± 0.2 klux) intensity at 25 ± 1°C temperature for a period of 14 days followed by exposure to stress conditions (0.1% sodium chloride, 0.1% sodium chloride with 0.1% sodium bicarbonate and 0.1% sodium chloride with 4 mM sodium acetate) and incubated further for a period of 21 days under high light intensity (3.5 ± 0.2 klux). Among the different autotrophic media used, BG11 and Z8 media were found to be the best for biomass and carotenoid production. The data in the table shows 3.6 and 3.2 (g/L) biomass with a carotenoid content of 0.28 and 0.27% (w/w) in BG11 and Z8 media with 0.1% sodium chloride, respectively. Under these conditions, the carotenoid profile indicated violaxanthin (6-9%), lutein (79-84%), astaxanthin (3-8%), zeaxanthin (0.32-0.78%), and -carotene (1.75-2.14%), which are identified by mass spectrum. These results indicate that B. braunii culture can grow in a wide range of media and produce lutein as major carotenoid which can be enhanced under stress conditions.

Item Type: Article
Uncontrolled Keywords: B. braunii, microalga, carotenoids, autotrophic media, stress, HPLC, LC-MS
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 20 Mar 2015 10:50
Last Modified: 16 Feb 2018 04:07
URI: http://ir.cftri.com/id/eprint/11776

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