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Effect of dehydroabietylamine in angiogenesis and GSK3-b inhibition during wound healing activity in rats.

Paramesha, M. and Ramesh, C. K. and Venkatarangaiah, K. (2015) Effect of dehydroabietylamine in angiogenesis and GSK3-b inhibition during wound healing activity in rats. Medicinal Chemistry Research, 24 (1). pp. 295-303.

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Abstract

The present research evaluates the wound healing capacity of dehydroabietylamine, a diterpenoid isolated from methanolic leaves extract of Carthamus tinctorius L. (Asteraceae). The wound healing activity was screened by excision, incision, and dead space wound models on albino rats and for the angiogenic property using chick chorioallantoic membrane (CAM) assay. Significant increase in wound contraction rate, skin breaking strength, granuloma strength, and dry granuloma weights was observed as compared to control. The pro-healing action may be due to increasing in collagen deposition as well better alignment and maturation. In CAM assay, the dehydroabietylamine showed increased angiogenic zone formation in the developing embryos compared to normal embryos and evidenced pro-angiogenic activity of the active constituent. Further, in silico comparative docking of dehydroabietylamine, standard drug nitrofurazone and positive control CHIR_98014 to ATP-binding active pocket of glycogen synthase kinase3-b (GSK3-b) protein that consisted of Tyr216 as a catalytic residue, with residues Asn64, Gly65, Ser66, Phe67, Gly68, Val70, Lys85, Leu132, Val135, Asp181, Lys183, Gln185, Asn186, and Asp200 in the active pocket docked with dehydroabietylamine at the torsional degree of freedom 0.5 U with Lamarckian genetic algorithm showed the inhibition constant of 315.6 9 10-9. But the inhibition constant of nitrofurazone and CHIR_98014 was 34.76 9 10-6 and 4.64 9 10-6 respectively. Docking of dehydroabietylamine to GSK3-b protein involved in Wnt signaling pathway supported the research on wound healing property. The above said studies explore the role of dehydroabietylamine in wound healing activity.

Item Type: Article
Uncontrolled Keywords: Carthamus tinctorius L. Dehydroabietylamine CAM assay GSK3-b protein In silico analysis Wound healing
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Feb 2015 10:33
Last Modified: 23 Feb 2015 10:33
URI: http://ir.cftri.com/id/eprint/11737

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