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Development of a rapid method for determination of lignans content in sesame oil.

Bhatnagar, A. S. and Hemavathy, J. and Gopala Krishna, A. G. (2015) Development of a rapid method for determination of lignans content in sesame oil. Journal of Food Science and Technology, 52 (1). ISSN 0022-1155

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Abstract

A spectrophotometric method for the rapid estimation of total lignans present in sesame oil was developed based on ultraviolet (UV) absorption and specific extinction (E1% 1cm) value of sesamin and sesamol. The E1% 1cm value obtained at characteristic UV absorbance of reference standards sesamin (λ max at 288 nm) and sesamol (λ max at 297 nm) was used to calculate the amount of lignans in sesame oil. The E1% 1cm value of 230.1 for sesamol at 288 nm in hexane-chloroform (7:3, v/v) was similar to that of sesamin and hence these were used for development of UVabsorption method for quantitation of lignans content in sesame oils. HPLC analysis confirmed and correlated well (r=0.99) with the results obtained by the UV absorption method developed in the present study. UV method developed in the present study could be used for the rapid estimation of lignans in sesame oils on a routine basis and would be of great help to sesame oil industries in monitoring their oil quality.

Item Type: Article
Uncontrolled Keywords: Sesame oil . Oil soluble sesame lignans . Sesamin .Sesamol .Sesamolin . Specific extinction (E1% 1cm) values
Subjects: 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions: Lipid Science and Traditional Foods
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 09 Jan 2015 12:06
Last Modified: 09 Jan 2015 12:06
URI: http://ir.cftri.com/id/eprint/11698

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