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Structural analyses and immunomodulatory properties offructo-oligosaccharides from onion (Allium cepa).

Prasanna Kumar, V. and Harish Prashanth, K. V. and Venkatesh, Y. P. (2015) Structural analyses and immunomodulatory properties offructo-oligosaccharides from onion (Allium cepa). Carbohydrate Polymers, 117. pp. 115-122.

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Abstract

tOnion (Allium cepa) is an immune-boosting food rich in fructans. The major aim of this study is tocharacterize and investigate the immunomodulatory properties of onion fructo-oligosaccharides (FOS).FOS was isolated from onion bulbs by hot 80% ethanol extraction (yield: ∼4.5 g/100 g fw) followedby gel permeation chromatography. NMR of onion FOS revealed unusual �-d-Glc terminal residue atthe non-reducing end. TLC and ESI–MS analyses showed that onion FOS ranged from trisaccharidesto hexasaccharides. Onion FOS (50 �g/mL) significantly increased (∼3-fold) the proliferation of mousesplenocytes/thymocytes vs. control. Further, onion FOS enhanced (∼2.5-fold) the production of nitricoxide by peritoneal exudates cells (PECs) from Wistar rats; intracellular free radicals production andphagocytic activity of isolated murine PECs were also augmented. Our structural and in vitro resultsindicate that onion FOS comprising of tri- to hexasaccharide units belongs to inulin-type fructans, andpossess immunostimulatory activities towards murine lymphocytes and macrophages.

Item Type: Article
Uncontrolled Keywords: Fructo-oligosaccharides Immunomodulation Inulin Mitogenicity Onion Phagocytosis
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
Divisions: Dept. of Biochemistry
Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Oct 2014 10:07
Last Modified: 16 Oct 2014 10:07
URI: http://ir.cftri.com/id/eprint/11665

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