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Elicitors, SA and MJ enhance carotenoids and tocopherol biosynthesis and expression of antioxidant related genes in Moringa oleifera Lam. leaves.

Saini, R. K. and Harish Prashanth, K. V. and Nandini, P. Shetty and Giridhar, P. (2014) Elicitors, SA and MJ enhance carotenoids and tocopherol biosynthesis and expression of antioxidant related genes in Moringa oleifera Lam. leaves. Acta Physiologiae Plantarum, 36 (10). pp. 2695-2704.

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Abstract

Moringa oleifera Lam. leaves are rich source of carotenoids (provitamin A) and a-tocopherol (vitamin E), and there is a scope for their further enhancement, through elicitor mediation, thereby a great potential for addressing these vitamins deficiency. In the present study, we report the efficacy of foliar administration of biotic elicitors, carboxy-methyl chitosan and chitosan, and signaling molecules, methyl jasmonate (MJ) and salicylic acid (SA) for enhancement of major carotenoids and a-tocopherol. Highest a-tocopherol content of 49.7 mg/100 g FW was recorded upon foliar application of 0.1 mM SA after 24 h of treatment, which represented a 187.5 % increase in comparison to the untreated control. Similarly, a maximum of 52.6 mg/100 g FW lutein, and 21.8 mg/100 g FW bcarotene content were observed in leaves after 24 h of treatment with MJ, which represented a 54.0 and 20.3 % increase in comparison to the untreated control, respectively. Among the major genes of carotenoid biosynthetic pathway, the expression of lycopene b-cyclase (LCY-b) was maximum influenced after treatment with elicitors and signaling molecules, compared to phytoene synthase and phytoene desaturase, suggesting the LCY-b-mediated enhancement in the production of b-carotene in elicitor treated M. oleifera leaves. Enhanced production of atocopherol under respective elicitor treatment was further supported by 2.0–2.7 fold up-regulation of c-tocopherol methyl transferase, compared to untreated control. This is the first report on elicitor-mediated enhanced production of tocopherol and carotenoids in foliage of economically important food plant.

Item Type: Article
Uncontrolled Keywords: Signaling molecules Salicylic acid Methyl jasmonate Chitosan Lycopene b-cyclase (LCY-b) c- Tocopherol methyl transferase
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Meat Fish and Poultry Technology
Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Oct 2014 08:12
Last Modified: 16 Oct 2014 08:12
URI: http://ir.cftri.com/id/eprint/11653

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