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Catechin concentrates of garden tea leaves (Camellia sinensis L.): extraction/isolation and evaluation of chemical composition.

Pravin Vasantrao, Gadkari and Udaya Sankar, K. and Manohar, B. (2014) Catechin concentrates of garden tea leaves (Camellia sinensis L.): extraction/isolation and evaluation of chemical composition. Journal of the Science of Food and Agriculture, 94. pp. 2921-2928.

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Abstract

BACKGROUND: Solid–liquid (SLE) and liquid–liquid (LLE) extraction techniques were applied to extract catechins and caffeine from quick mechanically expelled tea leaf juice (QMETLJ) and freeze-dried (FD)-QMETLJ of Camellia sinensis L. The concentrates obtained were analyzed for total polyphenol content and antioxidant activity (DPPH• inhibition, FRAP and phosphomolybdenum assay). Catechins were identified and quantified using HPLC. RESULTS: Overall, 95% (v/v) ethanol was the best solvent system for extracting total polyphenols (355.26±23.68 to 457.89±28.94 g GAE kg−1 extractable solid yield (ESY)) and antioxidants (DPPH• inhibition, 16.97±0.52 to 20.83±3.11%; FRAP, 4.15±0.32 to 6.38±0.57 mmol TE g−1 ESY; Mo(V) reduction, 2.47±0.19 to 3.84±0.39 mmol AAE g−1 ESY) from FD-QMETLJ. Similarly, in LLE, ethyl acetate showed the best results for recovering polyphenols (960.52±7.89 g GAE kg−1 ESY) and antioxidants (DPPH• inhibition, 42.39±0.91%; FRAP, 11.39±0.83 mmol TE g−1 ESY; Mo(V) reduction, 6.71±1.14 mmol AAE g−1 ESY) fromQMETLJ. CONCLUSION: It was found that 95% ethanol can be used to increase the total polyphenols and antioxidants in extracts from FD-QMETLJ, while ethyl acetate can be effectively employed for concentrating catechins fromQMETLJ.

Item Type: Article
Uncontrolled Keywords: Camellia sinensis L.; solid–liquid extraction; liquid–liquid extraction; catechins; antioxidant activity
Subjects: 600 Technology > 07 Beverage Technology > 08 Tea
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Oct 2014 06:49
Last Modified: 16 Oct 2014 06:49
URI: http://ir.cftri.com/id/eprint/11648

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