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Reaction Kinetics of the Invertase from Yeast (S. Cerevisiae).

Srinivas, Sistla and Purnima Kaul, Tiku (2014) Reaction Kinetics of the Invertase from Yeast (S. Cerevisiae). Food Biophysics, 9 (2). pp. 179-183.

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Abstract

Invertase converts sucrose to glucose and fructose. The reaction mechanism for the formation of glucose and fructose was studied by stopped flow spectrophotometer and circular dichroism. The reaction mechanism follows biphasic mode with rate constants of k10.0053 s−1±0.001 s−1 and k2 0.030 s−1±0.01 s−1 for 25 mM concentration of sucrose. Far UV circular dichroic spectrum of invertase in presence of sucrose shows 18 % increase in β conformation as a function of time. Taken together, the invertase hydrolysis follows biphasic mode where it undergoes conformational changes followed by hydrolysis of the sucrose.

Item Type: Article
Uncontrolled Keywords: Invertase . Sucrose . Glucose oxidase . Peroxidase . Stopped flow . Circular dichroism
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 May 2014 04:51
Last Modified: 28 May 2014 04:51
URI: http://ir.cftri.com/id/eprint/11576

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