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Detoxification Of Aflatoxin By Aqueous Extract Of Edible Plant Parts And Basidiomycetes Fungi.

Sayali Sudhakar, Deokar (2013) Detoxification Of Aflatoxin By Aqueous Extract Of Edible Plant Parts And Basidiomycetes Fungi. [Student Project Report] (Submitted)

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Abstract

In the present study an attempt has been made to screen plants/plant parts and fungi (basidiomyces) aqueous extracts for the ability to degrade AFB1, and to develop a molecular method through which aflatoxin detoxification can be detected. Aqueous extracts obtained from plants, plant parts and basidiomycetes fungi were evaluated for their ability to degrade aflatoxin B1 by thin-layer chromatography and competitive indirect enzyme-linked immunosorbent assay (ciELISA). Of the various plant extracts, the extract of Mint (Mentha arvensis) showed the maximum degradation up to 50 %. Followed by Brahmi (Centella asiatica). Extracts of yucca (Calotropis gigentea) and soybean (Glycine max) seeds showed shift in mobility of AFB1 on TLC. Among the eight aqueous extracts of basidiomycetes fungi analyzed, Armillaria sp. and Ganoderma sp. showed complete degradation as analyzed by TLC and ELISA. A molecular method for the detection of AFB1 detoxification is under progress. Initially AFB1 was treated with λDNA in the presence of S9 ezymes. Further AFB1-DNA adduct was purified and further treated with various restriction enzymes. Restriction pattern was analyzed for control λDNA and AFB1-DNA adducts λDNA. Initial studies showed that, restriction enzyme Hae III, Alu I and Bam H1 were non sensitive to AFB1-DNA adduct, hence they showed normal kind of restriction pattern. Not I was not having any restriction site in λDNA and Sma I, being a methylation sensitive enzyme, showed varied results. Screening of AFB1-DNA adduct sensitive restriction enzymes is under progress.

Item Type: Student Project Report
Uncontrolled Keywords: plants/plant parts, fungi, basidiomyces aqueous extracts, aflatoxin B1
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 03 Fungi
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Apr 2014 05:28
Last Modified: 30 Apr 2014 05:28
URI: http://ir.cftri.com/id/eprint/11530

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