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Immunodetection of Organochlorine Pesticide Endosulfan.

Rashmi, S. Shenoy (2013) Immunodetection of Organochlorine Pesticide Endosulfan. [Student Project Report] (Submitted)

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Abstract

Endosulfan, a derivative of hexachlorocyclopentadiene is one of the highly persistent organochlorine insecticides used worldwide and its residues have been found in the environment with its entry in the foods through food chain .Endosulphan and its metabolites have been reported to be highly toxic to aquatic fauna like fish and invertebrates. The acute, chronic toxicity and environmental concerns have attracted scientists for an effective and economically viable method for endosulfan detection. Currently, analysis of endosulfan is carried out using gas chromatography (GC) with electron capture detection (ECD). The equipment required is comparatively expensive, the analysis consumes large amounts of solvent, requires a stable and continuous electricity supply and high-purity gases. Also, samples need to be carefully cleaned up prior to analysis. With the increasing demand for pesticide residue analysis certification in export, there is a need to develop simple, specific, faster, cost effective, and sensitive assays to detect pesticide residues. Objective of the present study was to develop immunoassay for the detection of endosulfan. Immunoassays have benefits of being inexpensive, rapid and can be performed on simple, inexpensive equipment comparable to that by chromatographic methods. Chicken egg yolk immunoglobulins (IgY) from immunized hens have considerable advantages for the production of monoclonal antibodies because of the phylogenetic distance between birds and mammals and non-invasive collection. Endosulfan hapten was conjugated with BSA and poultry were immunized using Freunds complete adjuvant. Booster doses were given using Freunds incomplete adjuvant with 15 days intervals. The IgY antibodies were isolated from egg yolk by PEG -6000 method. Protein and antibody titre analysis indicated that protein was maximum on seventh week and antibody titre was maximum with third week egg. Checker board analysis indicated antigen dilution of 1:25000 and antibody dilution of 1:25000 were optimum for immune assay. Competitive direct ELISA using different concentrations of endosulfan indicated lower detection limit of 1ng for - and β- endosulfan respectively. Matrix effect was evaluated using few food samples spiked with endosulfan.

Item Type: Student Project Report
Uncontrolled Keywords: organochlorine insecticides, Endosulfan, chronic toxicity
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
600 Technology > 01 Medical sciences > 17 Toxicology
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 29 Apr 2014 11:07
Last Modified: 29 Apr 2014 11:07
URI: http://ir.cftri.com/id/eprint/11518

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