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Production and Optimization of Protease Enzyme from Aspergillus terreus CJS-127 using Jatropha Seed Cake.

Ajeeta, Anand (2013) Production and Optimization of Protease Enzyme from Aspergillus terreus CJS-127 using Jatropha Seed Cake. [Student Project Report] (Submitted)

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Abstract

Soil samples from in and around Mysore and Surathkal were collected for screening of fungi. The screening of fungi was done at three different pH 5.0, 7.0 and 9.0 using Czapek dox agar. The total microbial load was in the range of 4x104 CFU/g at pH 5.0 to 2.14x108 CFU/g at pH 9.0. The fungal load at pH 5.0 was in the range of 5x103-6.7x107 CFU/g. The fungal load at pH 7.0 was in the range of 1x105-9.4x107 CFU/g and the fungal load at pH 9.0 was in the range of 9x105-8.4x107CFU/g. Maximum load of fungi was in the samples screened at pH 5.0. The isolated fungi were screened for protease production and Aspergillus terreus CJS-127 was selected for optimization of protease production. The optimization of protease production was done by O-FAT method by selecting 5 parameters. Based on O-FAT result the maximum protease production was 59,515 U/g, on 5 days of incubation, at pH 7.0, with inoculum of 107 spores/ml, at 60% moisture content and at 30°C. Addition of external carbon and nitrogen sources to Jatropha seed cake did not enhance the protease production. Further optimization of protease production was done by Taguchi’s method. Taguchi is a wellknown technique that provides systematic and efficient methodology for design optimization. The primary advantages for design of experiments using Taguchi’s technique include simplification of experimental plan and feasibility of study of interaction between different parameters. The cultivation conditions optimized for protease production by Taguchi method were incubation period- 4 days, inoculum size- 107 spores/ml, moisture content- 50% and incubation temperature- 25°C. Under these optimized conditions, the maximum protease production was 1,02,832 U/g.

Item Type: Student Project Report
Uncontrolled Keywords: Protease, Aspergillus terreus, Optimization, OFAT and Taguchi’s method
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Apr 2014 06:35
Last Modified: 28 Apr 2014 06:35
URI: http://ir.cftri.com/id/eprint/11478

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