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Prebiotic oligosaccharides: Fermentative Production, evaluation of its bioactive potential and prebiotic efficacy using selected probiotic strains.

Mrudula, C. M. (2013) Prebiotic oligosaccharides: Fermentative Production, evaluation of its bioactive potential and prebiotic efficacy using selected probiotic strains. [Student Project Report] (Submitted)

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There is currently much interest in the concept of active management of the colonic microflora with the aim of improving the host health. This is traditionally attempted by the consumption of live microbial food supplements, known as `probiotics'. An alternative approach is the consumption of food ingredients known as prebiotics. The present study aims to evaluate the in vitro fermentative characteristics of prebiotic oligosaccharides using Bifidobacteria. The study intends to fill the existing gaps in the field of prebiotic research regarding the bifidogenic properties of prebiotic oligosaccharides using probiotic Bifidobacterium sp. the study might also contribute to study the prebiotic efficacy of NDOs with respect to bifidobacteria, to evaluate as to which NDOs can be used in the formulation of Infant formulas (as the numbers of bifidobacteria is higher in the infant GIT), in the production of synbiotics using Bifidobacteria and NDOs, for bioactivity studies of NDOs. Microbial production of oligosaccharides was done using crude fungal enzymes from A. oryzae and A. niger. FOS, GOS and IMO were the prebiotic oligosaccharides used for the current study. The prebiotic efficacy of the oligosaccharides was evaluated by measuring the increase in the numbers of bifidobacteria in vitro upon the supplementation of the oligosaccharides as the sole carbon source in the medium. The bifidobacterial cultures used for the study were Bifidobacterium bifidum and Bifidobacterium adolescentis. Fermented culture broth was centrifuged and the supernatant, without further purification will be used as the source of SCFA. Bioactivity studies such as Anti microbial and Anti oxidant activity assays were performed in postbiotics. It was found that 54% of FOS, 20% of GOS and 37% IMO was produced. Among the carbohydrates tested for prebiotic efficacy, GOS and IMO proved to be bifidogenic while FOS and Raftilose P95 supported fairly good growth of Bifidobacteria. The maximum growth rate of B. bifidum is much more than B. adolescentis on any carbohydrate source. Postbiotic studies of Bifidobacteria revealed that Bifidobacteria can be used as effective probiotics and the production of butyrate and can be implicated in anti microbial activities. Postbiotics can also be a good source of extracellular enzymes and depict anti oxidant activities.

Item Type: Student Project Report
Uncontrolled Keywords: prebiotic oligosaccharides, Bifidobacteria, Bifidobacterium
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 03 Apr 2014 11:48
Last Modified: 03 Apr 2014 11:48
URI: http://ir.cftri.com/id/eprint/11441

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