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Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators.

Namitha, K. K. and Negi, P. S. (2013) Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators. Notulae Scientia Biologicae, 5 (2). pp. 220-225.

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Abstract

A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill.) cultivar ‘Arka Ahuti’ was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones 6-Benzylamino purine (2 to 4 mg/L) and Indole-3-acetic acid (0.1 to 1 mg/L). The medium supplemented with 2 mg/L 6-benzylamino purine and 0.1 mg/L indole-3-acetic acid was found to be the best for inducing direct shoot regeneration and multiple shoots per explant from hypocotyl explants. Callus induction was observed in all the explants and regeneration of shoots was also promoted by all these combinations. Shoots were transferred to the elongation medium which also induced 100% rooting. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established for ‘Arka Ahuti’ cultivar of tomato for obtaining direct regeneration using hypocotyl, leaf and cotyledon as explants.

Item Type: Article
Uncontrolled Keywords: BAP, cotyledon, IAA, hypocotyl explant, regeneration
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
600 Technology > 08 Food technology > 23 Vegetables > 03 Tomato
Divisions: Fruit and Vegetable Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Jul 2013 04:26
Last Modified: 23 Jul 2013 04:26
URI: http://ir.cftri.com/id/eprint/11223

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