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Statistical Optimization of Bioprocess Parameters for Enhanced Gallic Acid Production from Coffee Pulp Tannins by Penicillium verrucosum.

Roopali, N. Bhoite and Navya, P. N. and Pushpa, S. Murthy (2013) Statistical Optimization of Bioprocess Parameters for Enhanced Gallic Acid Production from Coffee Pulp Tannins by Penicillium verrucosum. Preparative Biochemistry & Biotechnology, 43. pp. 350-363.

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Abstract

Gallic acid (3,4,5-trihydroxybenzoic acid) was produced by microbial biotransformation of coffee pulp tannins by Penicillium verrucosum. Gallic acid production was optimized using response surface methodology (RSM) based on central composite rotatable design. Process parameters such as pH, moisture, and fermentation period were considered for optimization. Among the various fungi isolated from coffee by-products, Penicillium verrucosum produced 35.23 lg=g of gallic acid on coffee pulp as sole carbon source in solid-state fermentation. The optimum values of the parameters obtained from the RSM were pH 3.32, moisture 58.40%, and fermentation period of 96 hr. Gallic acid production with an increase of 4.6-fold was achieved upon optimization of the process parameters. The results optimized could be translated to 1-kg tray fermentation. High-performance liquid chromatography (HPLC) analysis and spectral studies such as mass spectroscopy (MS) and 1H-nuclear magnetic resonance (NMR) confirmed that the bioactive compound isolated was gallic acid. Thus, coffee pulp, which is available in enormous quantity, could be used for the production of value-added products that can find avenues in food, pharmaceutical, and chemical industries.

Item Type: Article
Uncontrolled Keywords: coffee pulp, gallic acid, Penicillium verrucosum, response surface methodology, solid-state fermentation
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 07 Beverage Technology > 04 Coffee
Divisions: Plantation Products Spices and Flavour Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Apr 2013 09:46
Last Modified: 30 Jun 2015 12:39
URI: http://ir.cftri.com/id/eprint/11206

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