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In vitro propagation of Rivina humilis L. through proliferation of axillary shoots and shoot tips of mature plants.

Sri Harsha, P. S. C. and Imtiyaj Khan, Mohammad and Giridhar, P. and Ravishankar, G. A. (2012) In vitro propagation of Rivina humilis L. through proliferation of axillary shoots and shoot tips of mature plants. Indian Journal of Biotechnology. pp. 481-485. ISSN 0972-5849

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Abstract

In vitro propagation of Rivina humilis L. was attempted through proliferation of axillary shoots and shoot tips obtained from mature plants. The shoot tips and nodal shoot segments exhibited 70 and 80% shoot initiation when cultured on Murashige and Skoog (MS) basal medium supplemented with 4.44 µM 6-benzylaminopurine (BAP)+2.85 µM indole-3-acetic acid (IAA) and 8.88 µM BAP+2.68 µM naphthalene acetic acid (NAA), respectively. Shoots (2 to 3) were obtained from both shoot tip and nodal explants on MS medium supplemented with BAP (2.22 µM), IAA (2.85 µM) and silver nitrate (4 mM AgNO3). Multiplication was maximum on MS medium supplemented with BAP (8.88 µM), IAA (2.85 µM) and AgNO3 (4 mM) wherein 3-4 shoots per nodal explant were obtained. Microshoot elongation was achieved on MS medium containing BAP (2.22 µM) and gibberellic acid (2.89 µM GA3). Shoots (3-4 cm) exhibited 100% rooting within 4 wk in medium containing half MS with IAA (2.85-5.71 µM) or IBA (2.45-4.90 µM) alone or in combination with BAP (2.22 µM), resulting in simultaneous rooting and shoot growth. About 70% of micropropagated plants were established successfully in micropots and transferred to the field after 3 months.

Item Type: Article
Uncontrolled Keywords: In vitro rooting Rivina humilis Phytolaccaceae Multiple shoots Nodal explants
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Mar 2013 04:05
Last Modified: 11 Oct 2018 08:34
URI: http://ir.cftri.com/id/eprint/11159

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