[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Detection and identification of genetically modified EE-1 brinjal (Solanum melongena) by single, multiplex and SYBR real-time PCR.

Rajashekhar, V. Ballari and Asha, Martin and Gowda, Lalitha R. (2013) Detection and identification of genetically modified EE-1 brinjal (Solanum melongena) by single, multiplex and SYBR real-time PCR. Journal of the Science of Food and Agriculture, 93 (2). pp. 340-347. ISSN 0022-5142

[img] PDF
J Sci Food Agric (2012).pdf - Published Version
Restricted to Registered users only

Download (273kB)

Abstract

BACKGROUND: Brinjal is an important vegetable crop. Major crop loss of brinjal is due to insect attack. Insect-resistant EE-1 brinjal has been developed and is awaiting approval for commercial release. Consumer health concerns and implementation of international labelling legislation demand reliable analytical detectionmethods for genetically modified (GM) varieties. RESULTS: End-point and real-time polymerase chain reaction (PCR) methods were used to detect EE-1 brinjal. In end-point PCR, primer pairs specific to 35S CaMV promoter, NOS terminator and nptII gene common to other GM crops were used. Based on the revealed 3� transgene integration sequence, primers specific for the event EE-1 brinjal were designed. These primers were used for end-point single, multiplex and SYBR-based real-time PCR. End-point single PCR showed that the designed primers were highly specific to event EE-1 with a sensitivity of 20 pg of genomic DNA, corresponding to 20 copies of haploid EE-1 brinjal genomic DNA. The limits of detection and quantification for SYBR-based real-time PCR assay were 10 and 100 copies respectively. CONCLUSION: The prior development of detection methods for this important vegetable crop will facilitate compliance with any forthcoming labelling regulations.

Item Type: Article
Uncontrolled Keywords: genetically modified organisms; cry1Ac gene; event-specific PCR; Bt brinjal
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
600 Technology > 08 Food technology > 23 Vegetables
Divisions: Food Safety Analytical Quality Control Lab
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Nov 2012 10:44
Last Modified: 28 Jun 2016 08:27
URI: http://ir.cftri.com/id/eprint/11019

Actions (login required)

View Item View Item