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Development of a sensitive and specific immunoassay to evaluate diabetic nephropathy and dietary modulation in experimental animals.

Mallikarjun, B. C. and Smitha, B. and Shylaja, M. Dharmesh and Rajan, M. G. R. and Salimath, P. V. (2012) Development of a sensitive and specific immunoassay to evaluate diabetic nephropathy and dietary modulation in experimental animals. Journal of Pharmacy Research, 5 (7). pp. 3613-3617.

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Abstract

Microalbuminuria, is commonly associated with the development of diabetic nephropathy (DN) in type 1 diabetes. An increase in albumin excretion rate leading to microalbuminuria is widely recognized as the earliest indicator of diabetic nephropathy and as a risk factor for the development of obvious renal and macrovascular complications. The current study was aimed at the development of a sensitive and specific immunoassay for Rat Serum Albumin (RSA) so that many nutraceutics can be screened and validated in experimental rats for their potential use as anti-DN nutraceutics in humans. PAb-RSA was generated, developed radioimmunoassay and ELISA that could detect as low as ~1 μg of RSA in the urine/serum and applicability was examined by evaluating the efficacy of dietary components - Curcumin and Quercetin, in offering protection against diabetic nephropathy. Curcumin and quercetin were found to be effective in amelioration of fasting blood glucose, urinary volume, urinary sugar and glomerular filtration rate. The results were compared with urinary albumin levels as estimated by RIA. A correlation coefficient (R2) ~ 0.95 - 0.97 was observed suggesting the possible use of these assays in monitoring of DN state as well as anti-DN nutraceutic’s potency in experimental rat model.

Item Type: Article
Uncontrolled Keywords: Microalbuminuria, Curcumin, Quercetin, Radioimmunoassay, Diabetic nephropathy
Subjects: 600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Nov 2012 06:34
Last Modified: 30 Dec 2016 08:37
URI: http://ir.cftri.com/id/eprint/11014

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