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Determination of toxigenic potential of Fusarium species occurring on sorghum and maize grains produced in Karnataka, India by using Thin Layer Chromatography.

Sreenivasa, M. Y. and Diwakar, B. T. and Charith Raj, Adkar Purushothama (2012) Determination of toxigenic potential of Fusarium species occurring on sorghum and maize grains produced in Karnataka, India by using Thin Layer Chromatography. International Journal of Life Sciences, 6 (1). pp. 31-36.

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Abstract

In the present study, the fumonisin producing ability of Fusarium species from different geographic regions of Karnataka was investigated. Sorghum and maize samples were examined for the presence of Fusarium species. Mycological studies confirmed the occurrence of Fusarium species such as Fusarium verticillioides (18), F. proliferatum (2) and F. anthophilum (2) isolates. Efficacy to produce fumonisins on maize patties was carried out by using thin-layer chromatography. Fumonisin B1 and B2 were detected as a red-purple spots with an Rf value of 6.1 and 6.3 respectively. The TLC analysis showed that 17 of 18 F. verticillioides isolates, 02 of 02 F. proliferatum isolates and 01 of 02 F. anthophilum isolates produced fumonisins. On the chromatograms, identical colored spots for standards and samples with same Rf value were detected. Early detection of toxigenic Fusarium species is important to prevent the entry of these toxic substances into the food chains.

Item Type: Article
Uncontrolled Keywords: Fumonisin; Fusarium sp.; Maize; mycotoxin; Sorghum
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
600 Technology > 08 Food technology > 22 Legumes-Pulses > 02 Maize
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Nov 2012 06:28
Last Modified: 04 Sep 2018 11:31
URI: http://ir.cftri.com/id/eprint/11013

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