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Nano RNA aptamer wire for analysis of vitamin B12.

Selvakumar, L. S. and Thakur, M. S. (2012) Nano RNA aptamer wire for analysis of vitamin B12. Analytical Biochemistry, 427. pp. 151-157.

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Abstract

A simple and stable RNA aptamer-based colorimetric sensor for the detection of vitamin B12 using gold nanoparticles (AuNPs) has been proposed. Vitamin B12 belongs to the B vitamin group and prevents pernicious anemia, which is caused by vitamin B12 deficiency. A highly stable RNA aptamer that binds to vitamin B12 was employed by structural modification of 20-hydroxyl group of ribose to 20-flouro in all pyrimidines indicated in lowercase in 35-mer aptamer (50 GGA Acc GGu GcG cAu AAc cAc cuc AGu GcG AGc AA 30). Aggregation of AuNPs was specifically induced by desorption of the vitamin B12 binding RNA aptamer from the surface of AuNPs as a result of the aptamer–target interaction, leading to the color change from red to purple. The level of detection of vitamin B12 was 0.1 lg/ml by successful optimization of the amount of the aptamer, AuNPs, salts, and stability of the aptamer. Analysis of vitamin B12 was carried out, and the observed recovery was 92 to 95.3% with a relative standard deviation in the range of 2.08 to 8.27%. The results obtained were compared with those of the ultraviolet–visible (UV–vis) spectrometry method. This colorimetric aptasensor is advantageous for on-site detection with the naked eye.

Item Type: Article
Uncontrolled Keywords: Biosensor, 2-Fluoro modified RNA aptamer Gold nanoparticles, Vitamin B12
Subjects: 600 Technology > 02 Engineering & allied operations
600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 27 Aug 2012 04:15
Last Modified: 22 Oct 2018 05:31
URI: http://ir.cftri.com/id/eprint/10953

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