[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods.

Sowmya, N. and Thakur, M. S. and Manonmani, H. K. (2012) Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods. Journal of Applied Microbiology, 113. pp. 106-113. ISSN 1364-5072

[img] PDF
Journal_of_Applied_Microbiology_2012_113_1_106-113.pdf - Published Version
Restricted to Registered users only

Download (358kB)

Abstract

Aims: The study describes the development of simple and rapid DNA extraction method in combination with loop-mediated isothermal amplification (LAMP) to detect enterotoxigenic Staphylococcus aureus in food samples. Methods and Results: In this study, isolation of genomic DNA of enterotoxigenic Staph. aureus from spiked milk, milk burfi, khoa, sugarcane juice and boiled rice was carried out by boiling the isolated sample pellets for 10 min with 1% Triton X-100. The isolated DNA was evaluated by polymerase chain reaction (PCR) and LAMP method. The LAMP was found to be 100 times more sensitive than PCR. The LAMP assay was very specific for Staph. aureus, and the presence of other contaminating bacterial DNAs and food matrix did not interfere or inhibit the LAMP assay. Conclusions: The template DNA extraction method developed in this study for food samples is simple, rapid and cost-effective. LAMP was found to be less sensitive to matrix effect of food, compared to PCR. Significance and Impact of the Study: The method is suitable for direct detection of Staph. aureus without any enrichment in contaminated food samples and hence finds its application in food safety analysis, in permutation with LAMP.

Item Type: Article
Uncontrolled Keywords: DNA, enterotoxins, food safety, LAMP, PCR, Staphylococcus aureus, Triton X-100.
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
600 Technology > 08 Food technology > 13 Quality control > 02 Safety
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 Aug 2012 07:17
Last Modified: 24 Sep 2018 11:15
URI: http://ir.cftri.com/id/eprint/10951

Actions (login required)

View Item View Item