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Alkaline Phosphatase Formation in Escherichia Coli Vitamin B12/Methionine Auxotroph.

Pradhan, D. S. and Rege, D. V. and Sreenivasan, A. (1962) Alkaline Phosphatase Formation in Escherichia Coli Vitamin B12/Methionine Auxotroph. Enzymologia, 24 (2). pp. 105-112.

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Alkaline phosphatase has been demonstrated to be strongly induced in Escherichia coli K12 by growing the cells in a medium containing low phosphate (1)). This phenomenon was suggested to be a mechanism of negative feed-back with phosphate serving as repressor of phosphatase formation although activation during growth in low phosphate medium was not precluded. While studying changes in the enzyme make-up of E. coli B12/ methionine mutant growing in phosphate-deficient medium, we had observed an increase in alkaline phosphatase and to some extent in ribonuclease activities. These observations prompted us to examine the nature of elaboration of these enzymes, more so because ribonuclease is reported to reside in ribosomes of E. coli in a latent form (2)). In this paper, evidence has been presented to show that appearance of alkaline phosphatase (phosphomonoesterase) activity is the result of de novo synthesis of the enzyme protein. The phenomenon of alkaline phosphatase formation has also been examined in relation to concomitant nucleic acid synthesis.

Item Type: Article
Uncontrolled Keywords: Alkaline phosphatase, Escherichia coli
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 18 Jul 2012 09:38
Last Modified: 18 Jul 2012 09:38
URI: http://ir.cftri.com/id/eprint/10896

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