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Effects of n-3 polyunsaturated fatty acids on the reacylation of arachidonic acid in peritoneal macrophages

Lokesh, B. R. and Kinsella, J. E. (1994) Effects of n-3 polyunsaturated fatty acids on the reacylation of arachidonic acid in peritoneal macrophages. Prostaglandins, Leukotrienes and Essential Fatty Acids, 51 (4). pp. 235-239. ISSN 0952-3278

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Abstract

Mouse peritoneal macrophages were prelabelled with [3H]AA and activated ex vivo with phorbol myristate acetate (PMA) in the presence or absence of added n-3 polyunsaturated fatty acids (n-3 PUFA). The n-3 PUFA did not affect the release of [3H]AA from stimulated macrophages. Added AA, enhanced, in a dose-dependent manner, the amount of free [3H]AA in the medium after activating the cells with PMA. Both n-3 PUFA and AA were actively acylated into the lipids of PMA activated macrophages, but the n-3 PUFA did not compete with reacylation of AA. In unstimulated cells, in the absence of PMA, n-3 PUFA effectively competed with AA for acylation into membrane phospholipids. These studies suggest that distinct pools of AA exist in macrophages.

Item Type: Article
Uncontrolled Keywords: n-3 polyunsaturated fatty acids , macrophages
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 22 Feb 2018 10:48
Last Modified: 22 Feb 2018 10:48
URI: http://ir.cftri.com/id/eprint/10816

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